Monoclonal antibody targeting the beta-barrel assembly machine of Escherichia coli is bactericidal. Massive antibody discovery used to probe structure-function relationships of the essential outer membrane protein LptD.

Effect of lipopolysaccharide structure on reactivity of antiporin monoclonal antibodies with the bacterial cell surface. Outer membrane permeability and antibiotic resistance. Permeability barrier of Gram-negative cell envelopes and approaches to bypass it. Antimicrobial resistance: tackling a crisis for the health and wealth of nations (WHO, 2014). Drugs for bad bugs: confronting the challenges of antibacterial discovery. The antibiotic resistance crisis: part 1: causes and threats. Molecular basis of bacterial outer membrane permeability. Transmembrane diffusion of some hydrophobic substances. Outer membrane of Salmonella typhimurium. This study examines the penetration of sugars into cell wall-defective cells and outer membrane vesicles and reveals that the outer membrane acts as a size-limiting diffusion barrier. Penetration of oligo- and polysaccharides into isolated outer membrane vesicles and cells with degraded peptidoglycan layer.

Outer membrane as a diffusion barrier in Salmonella typhimurium. Biological functions and biogenesis of secreted bacterial outer membrane vesicles. Cell-to-cell transfer of bacterial outer membrane lipoproteins. Outer membrane lipid secretion and the innate immune response to Gram-negative bacteria. Bacterial outer membrane vesicles and the host–pathogen interaction. Immune modulation by bacterial outer membrane vesicles. Uncoiling mechanics of Escherichia coli type I fimbriae are optimized for catch bonds. A lipoprotein/beta-barrel complex monitors lipopolysaccharide integrity transducing information across the outer membrane. Biofilms, flagella, and mechanosensing of surfaces by bacteria. Transmembrane domain of surface-exposed outer membrane lipoprotein RcsF is threaded through the lumen of beta-barrel proteins. Detecting envelope stress by monitoring beta-barrel assembly.

Molecular basis of bacterial outer membrane permeability revisited. Outer membrane lipoprotein biogenesis: Lol is not the end. Asymmetric localization of lipopolysaccharides on the outer membrane of Salmonella typhimurium. Growth of the stress-bearing and shape-maintaining murein sacculus of Escherichia coli. This review extensively covers the structure, composition, biogenesis and function of the bacterial cell envelopes of Gram-negative and Gram-positive bacteria.

Cell wall of gram negative bacteria